The Theory and Practice of A-PRF and i-PRF: A Preparation Protocol for Dentists. How to Prepare Quality PRF-Clots

Dear colleagues!

I invite you to my masterclass “The theory and practice of A-PRF and i-PRF: a preparation protocol for dentists". You can now use syringes to prepare A-PRF – no tubes required!

To sign up, please call +49 1577 7081557 or email:

If you still prefer A-PRF therapy to PRP, then do not rush to buy special test tubes. You can use conventional syringes.

In the arsenal of every successful modern implantologist should be tools that make it easier to reach new heights of professional excellence. A regular mastering of innovative techniques (including PRF) is increasingly becoming a matter of prestige for self-respecting specialists. 

Under PRF is usually meant a platelet-rich fibrin, which is obtained by the process of centrifugation of patient's own blood. In essence, PRF is a blood plasma clot without red blood cells (erythrocytes). The procedure of centrifugation is carried out on site at the dentist's office.

What are the areas of PFR application in dentistry?

The main indications for use of PFR clots may be:

  • sinus lifting;
  • bone and soft tissue augmentation surgery;
  • various periodontal problems;
  • post-extraction management of the patient;
  • acceleration of wound healing, regeneration of gums and skin.

How PRF works?

In the course of the week a PRF clot gradually discharges the following active substances:


contribute to bone synthesis and trigger the conversion of monocytes into macrophages thanks to the intense stimulation of predecessor cells


a signal protein, also called a vascular endothelial growth factor, that stimulates vasculogenesis and angiogenesis


a protein, a platelet growth factor, highly important for angiogenesis


a protein, called transforming growth factor beta, that controls proliferation, cell differentiation, as well as many other cellular functions


important for stimulation of tissue growth and the process of angiogenesis


as a powerful inhibitor of angiogenesis, thrombospondin affects the growth and adhesion of endothelial cells 


a protein, an insulin-like growth factor-1

BMP-2 and BMP-7

a complex bone morphogenetic proteins that affect osteoblasts, osteoclasts, chondroblasts and chondrocytes, causing their proliferation and differentiation, which ultimately leads to intensive bone growth


What are the varietiesof PRF?

Five basic varieties of PFR are distinguished. Below we describe and analyse these and other similar methods in detail.

RF is a common fibrin clot that has successfully been used in dental practice for over 15 years. For its preparation 20.0 ml syringes are required. Centrifugation takes approx. 8-12 minutes at a speed of 3000 rpm.

No test tubes required. A blood clot is obtained with a 20.0 ml syringe.

A-PRF (advanced). This type of fibrin clot is obtained in about 15 minutes at a speed of 2000 rpm (in our centrifuge).A lower centrifugation speed helps to obtain a higher concentration of growth factors in the clot because of their more uniform distribution. It should be noted that due to individual properties of the blood, this rate of centrifugation can cause a decrease in ESR in some patients. In this case, fibrin coagulation may occur prior to a complete settlement of red blood cells, which will result in the red blood cells residue in the prepared clot.
No test tubes required. A blood clot is obtained with a 20.0 ml syringe.

PRF (injection PRF). This is a blood serum (after centrifugation). It is converted into a clot, having been centrifuged in 20.0 ml syringes. Using our centrifuge, the process takes 3 minutes at a speed of 1500 rpm. This time is enough for some red blood cells from the upper part of the test tube to settle without the plasma thickening. It is this plasma that subsequently provides the i-PRF for injections. The plasma thickening time is between 10 minutes (in pure form) and 4 minutes (with the added PRF clot).

Sticky bone. This is basically an i-PRF analogue, except that this Japanese method does not include a blood plasma activator in the test tube. The centrifugation process is similar to a conventional PRF. This method allows to obtain a pure plasma, without any residues. It thickens entirely due to the fibrin contained, therefore, the thickening time is relatively longer than when using an activator.
No tubes required. A blood clot is obtained with a 20.0 ml syringe.

What test tubes are required to work with PRF?

A PRF centrifuge with the tilt angle of 90 degrees is the principal requirement.

What is  the principal difference between the discussed A-PRF, i-PRF, PRF etc. technologies and PRP?

PRP uses the blood plasma containing leukocytes and platelets whose kinins and other biologically active substances initiate the tissue regeneration. This is due to the presence of an anticoagulant and targeted blood filtration.

In A-PRF, i-PRF, PRF etc. technologies the anticoagulant is not added, therefore a classical process produces a serum and a clot containing leukocytes and platelets.

For this reason special tubes are not required, it is enough to use standard 20.0 ml syringes and an adapted centrifuge.

The original PRP process does require special Dr.Renat Akhmerov® test tubes. If, however, a cheaper type of autological blood technology is used, then tubes are not necessary. It is possible to use syringes instead, which fit an adapted centrifuge that is available for order.

To sign up, please call +49 1577 7081557 or email: